Figure 3 of Hollborn, Mol Vis 2018; 24:518-535.


Figure 3. Transcriptional regulation of the C9 gene in cultured human RPE cells. The mRNA level was determined with real-time RT–PCR analysis after stimulation of the cells for 2 h, 6 h, and 24 h (as indicated by the panels of the bars). A: Effects of CoCl2 (150 µM) on the expression levels of complement genes. B: Effects of cell culturing in 1% O2 on the expression levels of complement genes. C: Effects of CoCl2 (150 µM) and hyperosmotic medium (+ 100 mM NaCl) on the cellular level of C9 mRNA. D: Effects of oxidative stress induced by H2O2 (20 µM) on the expression levels of complement genes. E: The level of C9 mRNA was determined in cells stimulated with the following compounds: glucose (25 mM), thrombin (10 U/ml), the activated blood coagulation factor X (FXa; 1 U/ml), VEGF (10 ng/ml), PDGF-B (10 ng/ml), TGF-β1 (10 ng/ml), IL-1β (10 ng/ml), TNFα (10 ng/ml), arachidonic acid (AA; 5 µM), and prostaglandin E2 (PGE2; 10 ng/ml). The numbers of independent experiments using cell lines from different donors are indicated in or above the bars. Significant difference versus unstimulated control: *p<0.05.