Figure 3. Chitosan inhibits the production of lipid droplets in adipocyte differentiation medium. A: Chitosan reduced the quantities of the fat granules dyed by Oil Red O (200X magnification) in a dose-dependent pattern. Oil Red O was dissolved in absolute isopropanol to quantify the lipid droplets. B: Chitosan decreased the optical density (OD) values of the dyed cell lysates as shown with the spectrophotometric analyses (490 nm). Magnification = 100X. Scale bar = 50 μm. Data are presented as the mean ± standard deviation (SD), **p<0.01 and *p<0.05. The experiments in this figure were repeated three times, and similar results were obtained.