Figure 1. Effect of MgAT and TAU on NMDA-induced changes in nNOS expression. A: Microphotographs of retinal sections immunostained with nNOS antibodies showing the effect of MgAT and TAU on the NMDA-induced increase in the expression of nNOS. (20X). Green fluorescence shows nNOS expression stained with fluorescein isothiocyanate (FITC; shown by arrow) while blue fluorescence shows retinal nuclei stained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bar represents 50 μm. B: Quantitative estimation of the effect of MgAT and TAU on the NMDA-induced increase in the expression of nNOS is also represented graphically. Pre, Co, and Post indicate that MgAT/TAU were injected 24 h before, simultaneously, or 24 h after intravitreal administration of NMDA, respectively. *p<0.05 versus the PBS-treated group, **p<0.01 versus the PBS-treated group, ***p<0.001 versus the PBS-treated group, ###p<0.001 versus the NMDA-treated group, n=6; bars represent mean ± SD. NMDA: N-methyl-D-aspartic acid, MgAT: Magnesium acetyl taurate, TAU: Taurine, GCL: Ganglion cell layer.