Figure 5. Immunolocalization of FKRP in the 661W photoreceptor cell line. Cells were immunostained with specific antibodies against FKRP (A, B, E; green) and the Golgi marker GM130 (D, E; red). Nuclei stained with DAPI are shown in blue (B). Enlarged views in C and F correspond to boxed areas in B and E, respectively. FKRP immunoreactivity was found in the cytoplasm and nucleus of 661W cells, and colocalized with GM130 in the cytoplasm (E, F; yellow). Each bar equals 20 μm, except in C and F: 5 μm,