Figure 1 of Haro, Mol Vis 2018; 24:43-58.

Figure 1. Expression of FKTN and FKRP at the mRNA and protein levels in the mammalian retina and 661W photoreceptor cell line. A: Total RNA was extracted from the neural retina of the indicated species and 661W cells, and the expression of the mRNAs encoded by the two genes was detected by RT–PCR using specific primers. PCR products were verified by DNA sequencing. B: Immunoblotting analysis of fukutin and FKRP expression was performed on total protein extracts (100 μg/lane) of the neural retina of the indicated species and 661W cells. C: Immunoblotting analysis of total (T), cytoplasmic (C) and nuclear (N) protein fractions extracted from the mouse neural retina and 661W cells (50 μg/lane) was performed using antibodies against fukutin, FKRP, β-tubulin III (a cytoplasmic control protein), and lamin A/C (two nuclear control proteins). The sizes of the bands obtained are given on the right side of each panel.