Figure 2. Stability of metabolic measurements from hfRPE cells assayed on polyester inserts. Average percent of the initial (A) oxygen consumption rate (OCR) and (B) extracellular acidification rate (ECAR) values for three different human fetal RPE (hfRPE) lines measured over time; technical triplicates were performed for each line. C: The average initial OCR and ECAR measurements compared to the final measurements obtained for the OCR and the ECAR show no statistically significant change over time. Results are expressed as mean ± standard deviation. ns = not significant.