Figure 1. Method for assaying RPE cells cultured on Transwell polyester supports. A: Depiction with the dimensions of the triangular template used to guide the insert scoring and cutting. B: Image of the fine diamond scriber (left) and a reverse Sinskey ophthalmic hook (right). C: Image of a Transwell inverted basal side up and in view with examples of a scored and cutout insert (arrow) and the placement
of the template on the Transwell. D: Image taken from above of a human fetal RPE insert positioned in a miniplate well.