Figure 5. Matrix metalloproteinase-14 (MMP-14) expression in diabetic rat retinas. A: MMP-14 expression was determined by western blot analysis on lysates of diabetic (D) and nondiabetic control (C) rats. After determination of the intensity of the MMP-14 protein band, intensities were adjusted to those of β-actin in
each sample. Each measurement was performed at least three times. B: In addition, cell extracts were used to quantify the mRNA of MMP-14 by quantitative-RT-PCR. Results are expressed as mean
± standard deviation of 7-10 rats in each group. *The difference between the two means was statistically significant at 5%
level of significance.