Table 2 of
Alves, Mol Vis 2018; 24:353-366. Table 2 of
Alves, Mol Vis 2018; 24:353-366.
Table 2. Primer sequences.
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) | Amplicon size (bp) |
|---|---|---|---|
| Reference genes | |||
| Hprt | ATGGGAGGCCATCACATTGT | ATGTAATCCAGCAGGTCAGCAA | 77 |
| Ywhaz | CAAGCATACCAAGAAGCATTTGA | GGGCCAGACCCAGTCTGA | 76 |
| Rho | GCAACAGGAGTCGGCTACCA | GCATAGGGAAGCCAGCAGATC | 76 |
| Target genes | |||
| Tnf | CCCAATCTGTGTCCTTCT | TTCTGAGCATCGTAGTTGT | 90 |
| Il-1β | ATAGAAGTCAAGACCAAAGTG | GACCATTGCTGTTTCCTAG | 109 |
| NOS2 | AGAGACAGAAGTGCGATC | AGATTCAGTAGTCCACAATAGTA | 96 |
| Pou4fi | ACCCTGTTCGTTTCAAATAG | AGCGTTTATCCCTGGTAAT | 104 |
| Tspo | TGTATTCGGCCATGGGGTATG | GAGCCAGCTGACCAGTGTAG | 105 |
| Qualitative PCR | |||
| Actb | GCTCCTCCTGAGCGCAAG | CATCTGCTGGAAGGTGGACA | 75 |