Figure 1. Chronic HSu treatment does not affect tight junction proteins and BRB permeability in the retina. A: The permeability of the blood–retinal barrier (BRB) was assessed with the Evans blue dye method. The results are expressed as milligrams of Evans blue per grams of retina, and are presented as mean ± standard error of the mean (SEM). B: The content of tight junction proteins (claudin-5, occludin, and ZO-1) were analyzed with western blotting in total retinal extracts obtained from control and high sucrose (HSu)-treated animals. Representative images of immunoreactive protein bands are presented above the graphs, as well as the respective loading control (β-actin). The results are expressed as a percentage of the age-matched controls, and data are presented as mean ± SEM.