Figure 6. Resolvin D1 promotes diabetic regeneration of corneal nerves and restoration of mechanical sensation. A: A Cochet-Bonnet esthesiometer was used to test the mechanical sensitivity of the cornea in healthy, diabetic and 4 day resolvin D1 (RvD1)-treated diabetic mice without removal of the corneal epithelium (n=8). B: Two weeks after the removal of the corneal epithelium, the renewing corneas were harvested, and the regenerated corneal nerve fibers were examined with corneal whole-mount staining. C: The immunofluorescence intensity of nerve fibers 14 days after injury was calculated with ImageJ (n=4). D: The restored corneal mechanical sensitivity was examined 3, 7, 10 and 14 days after the removal of the corneal epithelium in healthy mice, diabetic mice, and diabetic mice treated with 4 day RvD1 (n=6). Data are given as the mean ± standard deviation (SD); *p<0.05, **p<0.01, ***p<0.001, n.s: not significant.