Figure 4. Resolvin D1 attenuates ROS accumulation and NADPH oxidase 2/4 expression. A: Generation of reactive oxygen species (ROS) and expression of NADPH oxidase 2/4 were examined with immunofluorescence staining in healthy, diabetic, and 4 day resolvin D1 (RvD1)-treated diabetic corneal epithelia. B: The corneal epithelia were harvested from healthy mice and diabetic mice with or without 4 day RvD1 treatment and then evaluated with western blotting to examine the protein levels of NADPH oxidase 2/4. C: The quantified data of the western blotting results are shown (n=4). Data are given as the mean ± standard deviation (SD); *p<0.05, ***p<0.001.