Table 1 of
Hossain, Mol Vis 2018; 24:218-230. Table 1 of
Hossain, Mol Vis 2018; 24:218-230.
Table 1. Oligonucleotides used for bisulfite PCR and pyrosequencing analysis.
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|---|---|---|---|---|---|
| hLINE1F | proprietary (Qiagen product # 970042) | LINE1 promoters | 146 | 50 | BS PCR |
| hLINE1R | proprietary (Qiagen product # 970042) | LINE1 promoters | 146 | 50 | BS PCR |
| hLINE1-seq | proprietary (Qiagen product # 970042) | LINE1 promoters | N/A | N/A | pyrosequencing |
| hPax6-F1 | TAGTTATAGGTYGGGTTAAGGAAGGTTAAA | PAX6 promoter | 248 | 58 | BS PCR |
| hPax6-R1 | Bio-AACCTACCCCAAAATTTAAATATCAA | PAX6 promoter | 248 | 58 | BS PCR |
| hPax6-seq1 | ATTAGTYGGYGTAGAGTTGTGTTTA | PAX6 promoter | N/A | N/A | pyrosequencing |
| hPde6b-F1 | TGGGAAGTTTTAGGGTTTGAGG | PDE6B promoter | 120 | 58 | BS PCR |
| hPde6b-R1 | Bio-AAAACCCTATCATCAACAAAATCTTTCTTA | PDE6B promoter | 120 | 58 | BS PCR |
| hPde6b-seq1 | TTTAGGGTTTGAGGAGA | PDE6B promoter | N/A | N/A | pyrosequencing |
| hRho-F3 | TTGAGTTGGGATTTTGGGATAGATAAG | RHO promoter | 241 | 58 | BS PCR |
| JhRho-R3 | Bio-TATAAAATAACCTCCCCCTCCT | RHO promoter | 241 | 58 | BS PCR |
| hRho-S3 | TTTGGTTTTTTTTAGAAGTTAATTA | RHO promoter | N/A | N/A | pyrosequencing |
| hRho-F4 | AGGGGTTTGTAAATAAATGTTTAATGA | RHO promoter | 258 | 56 | BS PCR |
| hRho-R4 | Bio-ACTTTCTAATTTATTCTCCCAATCTCT | RHO promoter | 258 | 56 | BS PCR |
| hRho-seq4-2 | ATTGGATGATTTTAGAGGT | RHO promoter | N/A | N/A | pyrosequencing |
| hRER-F2 | Bio-GTGGGTTAGTTTTGATTTAAGGTAT | RHO Enhancer | 284 | 58 | BS PCR |
| hRER-R2 | CCCAAAATTCCCAAATCTATCTACTCAA | RHO Enhancer | 284 | 58 | BS PCR |
| hRER-seq2-1 | ACAAAACCAATAAAATAAAACCTCT | RHO Enhancer | N/A | N/A | pyrosequencing |