Table 1 of Hossain, Mol Vis 2018; 24:218-230.


Table 1. Oligonucleotides used for bisulfite PCR and pyrosequencing analysis.

Primer Name Sequence (5′-3′) Region Analyzed Amplicon Size (bp) Annealing temp (°C) Application
hLINE1F proprietary (Qiagen product # 970042) LINE1 promoters 146 50 BS PCR
hLINE1R proprietary (Qiagen product # 970042) LINE1 promoters 146 50 BS PCR
hLINE1-seq proprietary (Qiagen product # 970042) LINE1 promoters N/A N/A pyrosequencing
hPax6-F1 TAGTTATAGGTYGGGTTAAGGAAGGTTAAA PAX6 promoter 248 58 BS PCR
hPax6-R1 Bio-AACCTACCCCAAAATTTAAATATCAA PAX6 promoter 248 58 BS PCR
hPax6-seq1 ATTAGTYGGYGTAGAGTTGTGTTTA PAX6 promoter N/A N/A pyrosequencing
hPde6b-F1 TGGGAAGTTTTAGGGTTTGAGG PDE6B promoter 120 58 BS PCR
hPde6b-R1 Bio-AAAACCCTATCATCAACAAAATCTTTCTTA PDE6B promoter 120 58 BS PCR
hPde6b-seq1 TTTAGGGTTTGAGGAGA PDE6B promoter N/A N/A pyrosequencing
hRho-F3 TTGAGTTGGGATTTTGGGATAGATAAG RHO promoter 241 58 BS PCR
JhRho-R3 Bio-TATAAAATAACCTCCCCCTCCT RHO promoter 241 58 BS PCR
hRho-S3 TTTGGTTTTTTTTAGAAGTTAATTA RHO promoter N/A N/A pyrosequencing
hRho-F4 AGGGGTTTGTAAATAAATGTTTAATGA RHO promoter 258 56 BS PCR
hRho-R4 Bio-ACTTTCTAATTTATTCTCCCAATCTCT RHO promoter 258 56 BS PCR
hRho-seq4-2 ATTGGATGATTTTAGAGGT RHO promoter N/A N/A pyrosequencing
hRER-F2 Bio-GTGGGTTAGTTTTGATTTAAGGTAT RHO Enhancer 284 58 BS PCR
hRER-R2 CCCAAAATTCCCAAATCTATCTACTCAA RHO Enhancer 284 58 BS PCR
hRER-seq2-1 ACAAAACCAATAAAATAAAACCTCT RHO Enhancer N/A N/A pyrosequencing