Figure 2. The expression levels of IFN-related inflammatory cytokines in neutrophils, lymphocytes, and macrophages after zymosan stimulation. Zymosan increased the mRNA expression levels of interferon (IFN)-related inflammatory cytokines (interleukin (IL)-1α, IL-6, IL-12, and IL-17; normalized to β-actin) as well as the pro- and mature protein levels of IL-1α (E and F), which peaked at 8 h in neutrophils (A), lymphocytes (B), macrophages (C), and A6(1) cells (D) after zymosan stimulation. The data are presented as the mean ± standard error of the mean (SEM) and represent three individual experiments with five samples. *, p<0.05; **, p<0.01; ***, p<0.001.