To determine if Pten was knocked down by our AAV treatment, we stained the retina for Pten (Red) in retinas injected with the AAV-GFP vector (control) or AAV-shPTEN-GFP to knock down Pten. In the control retina, all of the GFP positive cells were also well labeled for Pten. In the retinas that received the AAV-shPTEN-GFP, the transfected cells (GFP positive) had low levels of Pten staining indicating that the vector had in fact decreased the expression of Pten. To access the data, click or select the words “Appendix 2”