Figure 3. Quantitative western blot analyses. A: Histone H3 phosphorylated at serine 10 (H3.phospho) and B: histone H4 (H4) expression significantly reduced in Q344X retinas. Band intensities were normalized to histone H3 (H3ac) expression. C: Loading control histone H3 was used to normalize band intensity. D: Statistical analyses of protein expression level; both histone H3 phosphorylated at serine 10 and histone H4 are significantly reduced (p<0.05) based on a Student t test. n=5 per group, ±SEM, significance determined by a Student’s t test: *p<0.05.