Appendix 6 of Bales, Mol Vis 2018; 24:153-164.


Appendix 6. qRT-PCR on select differentially expressed genes.

Quantitative polymerase chain reaction on reverse-transcribed RNA (qRT-PCR) isolated from retinas of 3-week-old Q344X and WT mice. Downregulated genes tested were: potassium channel, subfamily V, member 2, Kcnv2 (p=0.0008); histone cluster 2 H4, Hist2H4 (p=0.032); histone cluster 3 H2a, Hist3h2a (p=0.017). Upregulated genes tested were: protease, serine 33, Prss33 (p=0.0023) and paired box 7, Pax7 (p=0.034). All genes tested were found expressed in the same direction of change (down or upregulated) matching the results found from our RNA-seq data. n=12 per group, ±SEM, significance determined by Student t test: *p<0.05. To access the data, click or select the words “Appendix 6