Supplementary data 1a. Predicted Wilt-type coding sequence.

   1 ATGCCAATCGTGATGGCCCGGGACCTGGAGGAAACAGCATCATCCTCAGAGGATGAGGAG   60
   1 ‑M‑‑P‑‑I‑‑V‑‑M‑‑A‑‑R‑‑D‑‑L‑‑E‑‑E‑‑T‑‑A‑‑S‑‑S‑‑S‑‑E‑‑D‑‑E‑‑E‑   20

  61 GTCATAAGTCAAGAGGATCATCCATGCATCATGTGGACTGGAGGCTGCAGGAGAATTCCA  120
  21 ‑V‑‑I‑‑S‑‑Q‑‑E‑‑D‑‑H‑‑P‑‑C‑‑I‑‑M‑‑W‑‑T‑‑G‑‑G‑‑C‑‑R‑‑R‑‑I‑‑P‑   40

 121 GTTTTGGTATTCCATGCCGACGCTATTCTTACAAAGGACAACAATATTAGAGTAATTGGA  180

Wild-type acceptor site (c.182-1_182-2)

  41 ‑V‑‑L‑‑V‑‑F‑‑H‑‑A‑‑D‑‑A‑‑I‑‑L‑‑T‑‑K‑‑D‑‑N‑‑N‑‑I‑‑R‑‑V‑‑I‑‑G‑   60

…CTTTAG

 181 GAACGTTATCATTTGTCTTATAAGATTGTACGAACGGACAGTCGCCTAGTACGCAGCATT  240
  61 ‑E‑‑R‑‑Y‑‑H‑‑L‑‑S‑‑Y‑‑K‑‑I‑‑V‑‑R‑‑T‑‑D‑‑S‑‑R‑‑L‑‑V‑‑R‑‑S‑‑I‑   80

 241 CTGACAGCCCATGGATTTCATGAAGTTCACCCAAGCAGCACTGACTATAACCTAATGTGG  300
  81 ‑L‑‑T‑‑A‑‑H‑‑G‑‑F‑‑H‑‑E‑‑V‑‑H‑‑P‑‑S‑‑S‑‑T‑‑D‑‑Y‑‑N‑‑L‑‑M‑‑W‑  100

 301 ACAGGATCCCACCTGAAGCCCTTCTTACTGCGCACCCTCTCTGAAGCACAAAAAGTTAAT  360
 101 ‑T‑‑G‑‑S‑‑H‑‑L‑‑K‑‑P‑‑F‑‑L‑‑L‑‑R‑‑T‑‑L‑‑S‑‑E‑‑A‑‑Q‑‑K‑‑V‑‑N‑  120

 361 CACTTTCCCAGGTCTTATGAACTTACCCGGAAGGACCGACTGTACAAAAACATTATTCGA  420
 121 ‑H‑‑F‑‑P‑‑R‑‑S‑‑Y‑‑E‑‑L‑‑T‑‑R‑‑K‑‑D‑‑R‑‑L‑‑Y‑‑K‑‑N‑‑I‑‑I‑‑R‑  140

 421 ATGCAGCATACACATGGATTCAAGGCTTTTCACATCCTCCCCCAGACCTTCCTCCTGCCA  480
 141 ‑M‑‑Q‑‑H‑‑T‑‑H‑‑G‑‑F‑‑K‑‑A‑‑F‑‑H‑‑I‑‑L‑‑P‑‑Q‑‑T‑‑F‑‑L‑‑L‑‑P‑  160

 481 GCTGAGTACGCGGAATTTTGTAATTCATATTCGAAGGACCGGGGACCTTGGATAGTAAAA  540
 161 ‑A‑‑E‑‑Y‑‑A‑‑E‑‑F‑‑C‑‑N‑‑S‑‑Y‑‑S‑‑K‑‑D‑‑R‑‑G‑‑P‑‑W‑‑I‑‑V‑‑K‑  180

 541 CCAGTGGCATCTTCAAGGGGGCGGGGCGTCTACCTGATCAACAATCCAAACCAGATCTCC  600
 181 ‑P‑‑V‑‑A‑‑S‑‑S‑‑R‑‑G‑‑R‑‑G‑‑V‑‑Y‑‑L‑‑I‑‑N‑‑N‑‑P‑‑N‑‑Q‑‑I‑‑S‑  200

 601 CTGGAAGAGAACATTTTGGTCTCCCGTTACATTAACAACCCCCTGCTCATAGATGATTTC  660
 201 ‑L‑‑E‑‑E‑‑N‑‑I‑‑L‑‑V‑‑S‑‑R‑‑Y‑‑I‑‑N‑‑N‑‑P‑‑L‑‑L‑‑I‑‑D‑‑D‑‑F‑  220

 661 AAGTTTGACGTGCGCCTCTATGTGCTCGTGACTTCCTATGATCCTCTTGTCATCTATCTC  720
 221 ‑K‑‑F‑‑D‑‑V‑‑R‑‑L‑‑Y‑‑V‑‑L‑‑V‑‑T‑‑S‑‑Y‑‑D‑‑P‑‑L‑‑V‑‑I‑‑Y‑‑L‑  240

 721 TATGAAGAAGGATTGGCTAGGTTTGCAACTGTGCGATATGATCAAGGAGCCAAGAACATT  780
 241 ‑Y‑‑E‑‑E‑‑G‑‑L‑‑A‑‑R‑‑F‑‑A‑‑T‑‑V‑‑R‑‑Y‑‑D‑‑Q‑‑G‑‑A‑‑K‑‑N‑‑I‑  260

 781 CGGAACCAGTTCATGCATCTGACAAACTACAGTGTCAACAAGAAAAGTGGAGATTACGTC  840
 261 ‑R‑‑N‑‑Q‑‑F‑‑M‑‑H‑‑L‑‑T‑‑N‑‑Y‑‑S‑‑V‑‑N‑‑K‑‑K‑‑S‑‑G‑‑D‑‑Y‑‑V‑  280

 841 AGTTGTGACGATCCAGAAGTGGAGGATTATGGAAACAAATGGAGCATGAGTGCTATGCTT  900
 281 ‑S‑‑C‑‑D‑‑D‑‑P‑‑E‑‑V‑‑E‑‑D‑‑Y‑‑G‑‑N‑‑K‑‑W‑‑S‑‑M‑‑S‑‑A‑‑M‑‑L‑  300

 901 AGGTACCTGAAACAAGAAGGCAGAGATACAACCGCATTGATGGCCCATGTAGAAGACCTG  960
 301 ‑R‑‑Y‑‑L‑‑K‑‑Q‑‑E‑‑G‑‑R‑‑D‑‑T‑‑T‑‑A‑‑L‑‑M‑‑A‑‑H‑‑V‑‑E‑‑D‑‑L‑  320

 961 ATCATTAAGACTATAATCTCTGCTGAACTAGCTATTGCTACTGCCTGTAAAACCTTTGTT 1020
 321 ‑I‑‑I‑‑K‑‑T‑‑I‑‑I‑‑S‑‑A‑‑E‑‑L‑‑A‑‑I‑‑A‑‑T‑‑A‑‑C‑‑K‑‑T‑‑F‑‑V‑  340

1021 CCTCATCGCAGCAGTTGTTTTGAACTCTATGGCTTTGACGTGCTCATAGATTCTACTCTG 1080
 341 ‑P‑‑H‑‑R‑‑S‑‑S‑‑C‑‑F‑‑E‑‑L‑‑Y‑‑G‑‑F‑‑D‑‑V‑‑L‑‑I‑‑D‑‑S‑‑T‑‑L‑  360

1081 AAGCCATGGTTGTTGGAAGTGAATCTCTCTCCTTCTTTGGCCTGTGATGCGCCTCTGGAC 1140
 361 ‑K‑‑P‑‑W‑‑L‑‑L‑‑E‑‑V‑‑N‑‑L‑‑S‑‑P‑‑S‑‑L‑‑A‑‑C‑‑D‑‑A‑‑P‑‑L‑‑D‑  380

1141 CTAAAGATTAAAGCCAGTATGATTTCAGATATGTTCACTGTTGTAGGATTTGTGTGCCAA 1200
 381 ‑L‑‑K‑‑I‑‑K‑‑A‑‑S‑‑M‑‑I‑‑S‑‑D‑‑M‑‑F‑‑T‑‑V‑‑V‑‑G‑‑F‑‑V‑‑C‑‑Q‑  400

1201 GATCCTGCCCAGCGGGCATCAACTCGGCCAATTTATCCCACCTTTGAGTCTTCCAGGCGA 1260
 401 ‑D‑‑P‑‑A‑‑Q‑‑R‑‑A‑‑S‑‑T‑‑R‑‑P‑‑I‑‑Y‑‑P‑‑T‑‑F‑‑E‑‑S‑‑S‑‑R‑‑R‑  420

1261 AACCCTTTCCAGAAACCTCAGCGTTGCCGTCCACTCTCTGCCAGTGATGCGGAAATGAAA 1320
 421 ‑N‑‑P‑‑F‑‑Q‑‑K‑‑P‑‑Q‑‑R‑‑C‑‑R‑‑P‑‑L‑‑S‑‑A‑‑S‑‑D‑‑A‑‑E‑‑M‑‑K‑  440

1321 AACCTCGTGGGCTCAGCCCGGGAGAAAGGGCCAGGGAAGTTGGGTGGTTCTGTGCTTGGT 1380
 441 ‑N‑‑L‑‑V‑‑G‑‑S‑‑A‑‑R‑‑E‑‑K‑‑G‑‑P‑‑G‑‑K‑‑L‑‑G‑‑G‑‑S‑‑V‑‑L‑‑G‑  460

1381 CTGTCAATGGAGGAGATCAAAGTTTTACGAAGGGTGAAGGAGGAGAATGATCGGCGAGGT 1440
 461 ‑L‑‑S‑‑M‑‑E‑‑E‑‑I‑‑K‑‑V‑‑L‑‑R‑‑R‑‑V‑‑K‑‑E‑‑E‑‑N‑‑D‑‑R‑‑R‑‑G‑  480

1441 GGATTTATTCGCATATTTCCTACATCTGAGACATGGGAAATATATGGGTCCTACCTCGAG 1500
 481 ‑G‑‑F‑‑I‑‑R‑‑I‑‑F‑‑P‑‑T‑‑S‑‑E‑‑T‑‑W‑‑E‑‑I‑‑Y‑‑G‑‑S‑‑Y‑‑L‑‑E‑  500

1501 CATAAGACCTCAATGAACTATATGCTGGCAACACGCCTCTTCCAGGACAGAATGACTGCT 1560
 501 ‑H‑‑K‑‑T‑‑S‑‑M‑‑N‑‑Y‑‑M‑‑L‑‑A‑‑T‑‑R‑‑L‑‑F‑‑Q‑‑D‑‑R‑‑M‑‑T‑‑A‑  520

1561 GATGGAGCGCCAGAATTGAAGATAGAGAGTCTGAATTCAAAGGCCAAGCTGCATGCTGCA 1620
 521 ‑D‑‑G‑‑A‑‑P‑‑E‑‑L‑‑K‑‑I‑‑E‑‑S‑‑L‑‑N‑‑S‑‑K‑‑A‑‑K‑‑L‑‑H‑‑A‑‑A‑  540

1621 CTTTACGAGAGGAAGCTCCTGTCTCTGGAGGTGCGAAAACGTAGACGACGGAGTAGCAGA 1680
 541 ‑L‑‑Y‑‑E‑‑R‑‑K‑‑L‑‑L‑‑S‑‑L‑‑E‑‑V‑‑R‑‑K‑‑R‑‑R‑‑R‑‑R‑‑S‑‑S‑‑R‑  560

1681 TTGAGGGCAATGAGGCCAAAATACCCAGTGATTACCCAACCAGCTGAAATGAATGTTAAA 1740
 561 ‑L‑‑R‑‑A‑‑M‑‑R‑‑P‑‑K‑‑Y‑‑P‑‑V‑‑I‑‑T‑‑Q‑‑P‑‑A‑‑E‑‑M‑‑N‑‑V‑‑K‑  580

1741 ACTGAGACAGAGAGTGAAGAGGAGGAAGAAGTCGCATTAGATAATGAAGATGAAGAACAG 1800
 581 ‑T‑‑E‑‑T‑‑E‑‑S‑‑E‑‑E‑‑E‑‑E‑‑E‑‑V‑‑A‑‑L‑‑D‑‑N‑‑E‑‑D‑‑E‑‑E‑‑Q‑  600

1801 GAGGCTTCCCAGGAGGAGTCTGCAGGATTTCTTAGAGAAAATCAAGCCAAATATACACCC 1860
 601 ‑E‑‑A‑‑S‑‑Q‑‑E‑‑E‑‑S‑‑A‑‑G‑‑F‑‑L‑‑R‑‑E‑‑N‑‑Q‑‑A‑‑K‑‑Y‑‑T‑‑P‑  620

1861 TCATTGACAGCTTTGGTAGAAAATACACCCAAAGAAAATTCCATGAAAGTTCGTGAATGG 1920
 621 ‑S‑‑L‑‑T‑‑A‑‑L‑‑V‑‑E‑‑N‑‑T‑‑P‑‑K‑‑E‑‑N‑‑S‑‑M‑‑K‑‑V‑‑R‑‑E‑‑W‑  640

1921 AATAATAAAGGTGGACACTGCTGCAAACTTGAGACTCAGGAGCTAGAGCCTAAATTTAAC 1980
 641 ‑N‑‑N‑‑K‑‑G‑‑G‑‑H‑‑C‑‑C‑‑K‑‑L‑‑E‑‑T‑‑Q‑‑E‑‑L‑‑E‑‑P‑‑K‑‑F‑‑N‑  660

1981 CTGATGCAGATTCTTCAAGATAATGGCAATCTTAGCAAAATGCAGGCCCGAATAGCATTC 2040
 661 ‑L‑‑M‑‑Q‑‑I‑‑L‑‑Q‑‑D‑‑N‑‑G‑‑N‑‑L‑‑S‑‑K‑‑M‑‑Q‑‑A‑‑R‑‑I‑‑A‑‑F‑  680

2041 TCTGCCTATCTCCAGCATGTTCAAATTCGCCTGATGAAAGACAGTGGCGGTCAGACGTTC 2100
 681 ‑S‑‑A‑‑Y‑‑L‑‑Q‑‑H‑‑V‑‑Q‑‑I‑‑R‑‑L‑‑M‑‑K‑‑D‑‑S‑‑G‑‑G‑‑Q‑‑T‑‑F‑  700

2101 AGTGCCAGTTGGGCTGCCAAAGAGGATGAACAGATGGAGCTGGTTGTTCGTTTCCTCAAG 2160
 701 ‑S‑‑A‑‑S‑‑W‑‑A‑‑A‑‑K‑‑E‑‑D‑‑E‑‑Q‑‑M‑‑E‑‑L‑‑V‑‑V‑‑R‑‑F‑‑L‑‑K‑  720

2161 CGAGCATCAAATAACCTCCAGCATTCACTGAGGATGGTATTACCCAGTCGACGATTGGCA 2220
 721 ‑R‑‑A‑‑S‑‑N‑‑N‑‑L‑‑Q‑‑H‑‑S‑‑L‑‑R‑‑M‑‑V‑‑L‑‑P‑‑S‑‑R‑‑R‑‑L‑‑A‑  740

2221 CTTCTGGAACGCAGAAGAATCCTGGCCCACCAGCTGGGTGACTTTATCATTGTATACAAC 2280
 741 ‑L‑‑L‑‑E‑‑R‑‑R‑‑R‑‑I‑‑L‑‑A‑‑H‑‑Q‑‑L‑‑G‑‑D‑‑F‑‑I‑‑I‑‑V‑‑Y‑‑N‑  760

2281 AAGGAAACAGAACAAATGGCTGAAAAGAAATCAAAGAAGAAAGTTGAGGAAGAAGAGGAA 2340
 761 ‑K‑‑E‑‑T‑‑E‑‑Q‑‑M‑‑A‑‑E‑‑K‑‑K‑‑S‑‑K‑‑K‑‑K‑‑V‑‑E‑‑E‑‑E‑‑E‑‑E‑  780

2341 GATGGGGTGAATATGGAAAACTTTCAGGAGTTCATCAGACAAGCAAGTGAGGCTGAACTG 2400
 781 ‑D‑‑G‑‑V‑‑N‑‑M‑‑E‑‑N‑‑F‑‑Q‑‑E‑‑F‑‑I‑‑R‑‑Q‑‑A‑‑S‑‑E‑‑A‑‑E‑‑L‑  800

2401 GAGGAGGTGTTGACTTTTTATACCCAAAAGAACAAGTCTGCTAGTGTCTTCCTGGGGACT 2460
 801 ‑E‑‑E‑‑V‑‑L‑‑T‑‑F‑‑Y‑‑T‑‑Q‑‑K‑‑N‑‑K‑‑S‑‑A‑‑S‑‑V‑‑F‑‑L‑‑G‑‑T‑  820

2461 CACTCTAAAATTTCTAAGAACAACAACAATTATTCTGATAGTGGGGCAAAAGGTGATCAC 2520
 821 ‑H‑‑S‑‑K‑‑I‑‑S‑‑K‑‑N‑‑N‑‑N‑‑N‑‑Y‑‑S‑‑D‑‑S‑‑G‑‑A‑‑K‑‑G‑‑D‑‑H‑  840

2521 CCTGAGACTATAATGGAAGAAGTGAAAATAAAGCCACCTAAACAGCAACAGACGACAGAA 2580
 841 ‑P‑‑E‑‑T‑‑I‑‑M‑‑E‑‑E‑‑V‑‑K‑‑I‑‑K‑‑P‑‑P‑‑K‑‑Q‑‑Q‑‑Q‑‑T‑‑T‑‑E‑  860

2581 ATTCATTCTGATAAATTATCTCGATTTACCACTTCAGCAGAAAAAGAGGCAAAATTAGTT 2640
 861 ‑I‑‑H‑‑S‑‑D‑‑K‑‑L‑‑S‑‑R‑‑F‑‑T‑‑T‑‑S‑‑A‑‑E‑‑K‑‑E‑‑A‑‑K‑‑L‑‑V‑  880

2641 TATAGCAATTCCTCCTCTGGTCCTACTGCTACTCTGCAGAAAATTCCCAACACCCATTTG 2700
 881 ‑Y‑‑S‑‑N‑‑S‑‑S‑‑S‑‑G‑‑P‑‑T‑‑A‑‑T‑‑L‑‑Q‑‑K‑‑I‑‑P‑‑N‑‑T‑‑H‑‑L‑  900

2701 TCATCTGTTACAACCTCTGACCTCTCTCCAGGGCCTTGCCACCATTCTTCTTTATCTCAA 2760
 901 ‑S‑‑S‑‑V‑‑T‑‑T‑‑S‑‑D‑‑L‑‑S‑‑P‑‑G‑‑P‑‑C‑‑H‑‑H‑‑S‑‑S‑‑L‑‑S‑‑Q‑  920

2761 ATTCCTTCAGCTATCCCCAGCATGCCTCACCAGCCAACAATTTTACTGAACACAGTCTCT 2820
 921 ‑I‑‑P‑‑S‑‑A‑‑I‑‑P‑‑S‑‑M‑‑P‑‑H‑‑Q‑‑P‑‑T‑‑I‑‑L‑‑L‑‑N‑‑T‑‑V‑‑S‑  940

2821 GCCAGTGCTTCTCCCTGCCTACATCCCGGGGCACAGAACATCCCAAGCCCTACTGGCCTG 2880
 941 ‑A‑‑S‑‑A‑‑S‑‑P‑‑C‑‑L‑‑H‑‑P‑‑G‑‑A‑‑Q‑‑N‑‑I‑‑P‑‑S‑‑P‑‑T‑‑G‑‑L‑  960

2881 CCACGCTGTCGATCAGGAAGTCACACCATTGGTCCCTTTTCTTCCTTCCAAAGTGCTGCA 2940
 961 ‑P‑‑R‑‑C‑‑R‑‑S‑‑G‑‑S‑‑H‑‑T‑‑I‑‑G‑‑P‑‑F‑‑S‑‑S‑‑F‑‑Q‑‑S‑‑A‑‑A‑  980

2941 CACATCTATAGCCAGAAACTGTCTCGTCCCTCTTCAGCAAAGGCAGGATCGTGCTATCTA 3000
 981 ‑H‑‑I‑‑Y‑‑S‑‑Q‑‑K‑‑L‑‑S‑‑R‑‑P‑‑S‑‑S‑‑A‑‑K‑‑A‑‑G‑‑S‑‑C‑‑Y‑‑L‑ 1000

3001 AACAAGCATCATTCAGGAATAGCCAAAACACAAAAAGAGGGAGAAGATGCTTCTTTATAT 3060
1001 ‑N‑‑K‑‑H‑‑H‑‑S‑‑G‑‑I‑‑A‑‑K‑‑T‑‑Q‑‑K‑‑E‑‑G‑‑E‑‑D‑‑A‑‑S‑‑L‑‑Y‑ 1020

3061 AGCAAACGGTACAACCAAAGTATGGTTACAGCTGAACTTCAGCGGCTAGCTGAGAAGCAG 3120
1021 ‑S‑‑K‑‑R‑‑Y‑‑N‑‑Q‑‑S‑‑M‑‑V‑‑T‑‑A‑‑E‑‑L‑‑Q‑‑R‑‑L‑‑A‑‑E‑‑K‑‑Q‑ 1040

3121 GCAGCGAGACAGTATTCTCCATCCAGCCACATCAACCTCCTCACCCAACAGGTAACAAAC 3180
1041 ‑A‑‑A‑‑R‑‑Q‑‑Y‑‑S‑‑P‑‑S‑‑S‑‑H‑‑I‑‑N‑‑L‑‑L‑‑T‑‑Q‑‑Q‑‑V‑‑T‑‑N‑ 1060

3181 CTGAATTTGGCAACTGGCATCATAAACAGAAGCAGTGCTTCAGCTCCCCCAACCCTCCGA 3240
1061 ‑L‑‑N‑‑L‑‑A‑‑T‑‑G‑‑I‑‑I‑‑N‑‑R‑‑S‑‑S‑‑A‑‑S‑‑A‑‑P‑‑P‑‑T‑‑L‑‑R‑ 1080

3241 CCCATCATCAGTCCTAGTGGCCCGACATGGTCTACACAGTCAGACCCCCAAGCTCCCGAG 3300
1081 ‑P‑‑I‑‑I‑‑S‑‑P‑‑S‑‑G‑‑P‑‑T‑‑W‑‑S‑‑T‑‑Q‑‑S‑‑D‑‑P‑‑Q‑‑A‑‑P‑‑E‑ 1100

3301 AATCACTCCAGCTCTCCTGGAAGCAGGAGCCTGCAGACAGGGGGATTTGCCTGGGAAGGA 3360
1101 ‑N‑‑H‑‑S‑‑S‑‑S‑‑P‑‑G‑‑S‑‑R‑‑S‑‑L‑‑Q‑‑T‑‑G‑‑G‑‑F‑‑A‑‑W‑‑E‑‑G‑ 1120

3361 GAAGTAGAAAACAACGTGTACAGCCAGGCTACAGGGGTGGTCCCCCAGCACAAGTATCAC 3420
1121 ‑E‑‑V‑‑E‑‑N‑‑N‑‑V‑‑Y‑‑S‑‑Q‑‑A‑‑T‑‑G‑‑V‑‑V‑‑P‑‑Q‑‑H‑‑K‑‑Y‑‑H‑ 1140

3421 CCCACAGCAGGCAGCTATCAGCTTCAATTTGCCCTGCAGCAACTTGAACAACAAAAACTT 3480
1141 ‑P‑‑T‑‑A‑‑G‑‑S‑‑Y‑‑Q‑‑L‑‑Q‑‑F‑‑A‑‑L‑‑Q‑‑Q‑‑L‑‑E‑‑Q‑‑Q‑‑K‑‑L‑ 1160

3481 CAGTCCCGGCAGCTCCTGGACCAGAGTCGAGCCCGGCACCAGGCAATCTTTGGCAGCCAG 3540
1161 ‑Q‑‑S‑‑R‑‑Q‑‑L‑‑L‑‑D‑‑Q‑‑S‑‑R‑‑A‑‑R‑‑H‑‑Q‑‑A‑‑I‑‑F‑‑G‑‑S‑‑Q‑ 1180

3541 ACACTACCTAACTCCAATTTATGGACAATGAATAATGGTGCAGGTTGTAGAATTTCCAGT 3600
1181 ‑T‑‑L‑‑P‑‑N‑‑S‑‑N‑‑L‑‑W‑‑T‑‑M‑‑N‑‑N‑‑G‑‑A‑‑G‑‑C‑‑R‑‑I‑‑S‑‑S‑ 1200

3601 GCCACAGCTAGTGGCCAGAAGCCAACCACTCTGCCACAAAAAGTGGTACCACCTCCAAGT 3660
1201 ‑A‑‑T‑‑A‑‑S‑‑G‑‑Q‑‑K‑‑P‑‑T‑‑T‑‑L‑‑P‑‑Q‑‑K‑‑V‑‑V‑‑P‑‑P‑‑P‑‑S‑ 1220

3661 TCTTGCGCCTCCCTGGTTCCCAAACCCCCACCCAACCACGAACAAGTGCTCAGAAGGGCA 3720
1221 ‑S‑‑C‑‑A‑‑S‑‑L‑‑V‑‑P‑‑K‑‑P‑‑P‑‑P‑‑N‑‑H‑‑E‑‑Q‑‑V‑‑L‑‑R‑‑R‑‑A‑ 1240

3721 ACATCCCAGAAAGCTTCCAAAGGGTCCTCCGCGGAAGGGCAGCTGAATGGACTCCAGAGC 3780
1241 ‑T‑‑S‑‑Q‑‑K‑‑A‑‑S‑‑K‑‑G‑‑S‑‑S‑‑A‑‑E‑‑G‑‑Q‑‑L‑‑N‑‑G‑‑L‑‑Q‑‑S‑ 1260

3781 AGCCTTAACCCTGCAGCCTTTGTGCCCATCACCAGCTCTACAGATCCTGCTCACACTAAA 3840
1261 ‑S‑‑L‑‑N‑‑P‑‑A‑‑A‑‑F‑‑V‑‑P‑‑I‑‑T‑‑S‑‑S‑‑T‑‑D‑‑P‑‑A‑‑H‑‑T‑‑K‑ 1280

3841 ATATGA                                                       3846
1281 ‑I‑‑*‑                                                       3846

Supplementary data 1b. Predicted mutated coding sequence.

   1 ATGCCAATCGTGATGGCCCGGGACCTGGAGGAAACAGCATCATCCTCAGAGGATGAGGAG   60
   1 ‑M‑‑P‑‑I‑‑V‑‑M‑‑A‑‑R‑‑D‑‑L‑‑E‑‑E‑‑T‑‑A‑‑S‑‑S‑‑S‑‑E‑‑D‑‑E‑‑E‑   20

  61 GTCATAAGTCAAGAGGATCATCCATGCATCATGTGGACTGGAGGCTGCAGGAGAATTCCA  120
  21 ‑V‑‑I‑‑S‑‑Q‑‑E‑‑D‑‑H‑‑P‑‑C‑‑I‑‑M‑‑W‑‑T‑‑G‑‑G‑‑C‑‑R‑‑R‑‑I‑‑P‑   40

 121 GTTTTGGTATTCCATGCCGACGCTATTCTTACAAAGGACAACAATATTAGAGTAATTGGA  180

Intronic Splice-site variant

New constituent acceptor site (c.203_204)

  41 ‑V‑‑L‑‑V‑‑F‑‑H‑‑A‑‑D‑‑A‑‑I‑‑L‑‑T‑‑K‑‑D‑‑N‑‑N‑‑I‑‑R‑‑V‑‑I‑‑G‑   60

…CTTAA

 181 GAACGTTATCATTTGTCTTATAAG-ATTGTACGAACGGACAGTCGCCTAGTACGCAGCAT  240

New exon start

  61 ‑E‑‑R‑‑Y‑‑H‑‑L‑‑S‑‑Y‑‑K‑‑D‑‑C‑‑T‑‑N‑‑G‑‑Q‑‑S‑‑P‑‑S‑‑T‑‑Q‑‑H‑   80

Lost coding sequence

Early Stop codon

 241 TCTGACAGCCCATGGATTTCATGA  						 264
  81 ‑S‑‑D‑‑S‑‑P‑‑W‑‑I‑‑S‑‑*-						  88