Figure 3. Inflammatory cells acquire a hypertrophic morphology and newly synthesized microglia increases in the ON following rNAION. Iba1 (Green) is a global inflammatory marker. ED1 (red) represents newly generated macrophages/microglia. A: Control (naïve) optic nerve (ON). Quiescent resident microglia are demonstrable as Iba1(+)/ED1(-) ramified structures (scale bar: 50 μm). Insets show images at lower magnification (200 μm). B: Anterior ON 3 day post-rodent nonarteritic anterior ischemic optic neuropathy (rNAION). The majority of Iba1-positive cells are now globoid (activated macrophages), and ED1-positive colocalization is present, indicating newly synthesized macrophages. C: Anterior ON section 30 days post-rNAION. There is strong Iba1/ED1-positive colocalization, and the anterior region is packed with inflammatory cells. D: Distal ON section 30 days post-rNAION.