Figure 4. NET analysis in the posterior chamber upon IL-8 or TNF-α injections. The sham-injected eyes were negative for neutrophil extracellular traps (NETs; A, D, G, J, M). Netting neutrophils and NETs were observed in the eyes injected with interleukin (IL)-8 and tumor necrosis factor alpha (TNF-α) and were based on colocalization of neutrophil elastase (NE, red; E, F, arrows), myeloperoxidase (MPO, green; H, I, arrows), and citrullinated histone 3 (H3Cit, purple; K, L, arrows). 4',6-diamidino-2-phenylindole (DAPI, blue; N, O, arrows) shows the nuclear morphology. Magnification = 63X. Scale bar = 50 μm.