Validation of neutrophil infiltration. Neutrophils infiltration was demonstrated by staining with Ly6G (red; panels A, B) and CD11b (green; panels C, D); merge images with DAPI for nuclear staining (blue) is shown in panels E, F. The figure shows staining of sham injected (A, C, E) and IL-8/TNFα -injected (B, D, F) eyes. Ganglion cell layer (GCL), vitreous (V). Representative images of 3 eyes (n=3/group), magnification x63, scale bar 20 μm. To access the data, click or select the words “Appendix 1”