Figure 4. Erk1/2 activation induced by retinal ischemia is reduced by azithromycin administration. A: Representative immunoblot showing the expression of phospho-p44/p42 MAPK (pERK1/2) at 1 or 6 h of reperfusion. B: Systemic treatment with azithromycin significantly reduced ERK1/2 phosphorylation after 6 h of reperfusion in the ischemic
eye (R) of azithromycin-treated rats compared to the vehicle-injected animals. Histograms show the results of densitometric
analysis of the bands, normalized to the value of actin, and expressed as mean ± standard error of the mean (SEM; n = 4 for
each group; **p<0.01, ***p<0.001; ANOVA followed by Tukey-Kramer test for multiple comparisons). AZM = azithromycin-treated
rats; VEH = vehicle-treated rats; Rep time = reperfusion time; R = right ischemic eye; L = left eye; MW = molecular weight.