Figure 2. Azithromycin prevents calpain activation and Bad upregulation following retinal ischemia/reperfusion. A: Immunoblotting showing the reduced accumulation of calpain-specific 150/145 kDa SBDP and (B) Bcl-2-associated death promoter (Bad) expression levels after 1 and 6 h of reperfusion in the ischemic retina of azithromycin-treated rats compared to vehicle-treated rats. Histograms show the results of densitometric analysis of the bands normalized to the value of actin and expressed as mean ± standard error of the mean (SEM; n = 4 for each group; *p<0.05, ***p<0.001; ANOVA followed by the Tukey-Kramer test for multiple comparisons). AZM = azithromycin-treated rats; VEH = vehicle-treated rats; Rep time = reperfusion time; R = right ischemic eye; L = left eye; MW = molecular weight.