Figure 5. Effects of fisetin on EGF-induced the expression of AKT and Sp1 in ARPE-19 cells. A: Western blotting of the nuclear fraction of Sp1 in epidermal growth factor (EGF; 20 ng/ml) or treated with EGF (20 ng/ml) plus fisetin (5 and 10 μM) for 24 h. B: Quantitative analysis of western blots. C: Western blotting for the expression of p-AKT, AKT, and Sp1. D: Sp1 activity was detected with the luciferase assay. Data are presented as the mean ± standard error of the mean (SEM) of at least three independent experiments. **p<0.01 compared with control cells; #p<0.01 compared with fisetin.