Figure 2. Effects of treatment with fisetin in EGF-induced MMP-2 and MMP-9 expression in ARPE-19 cells. A: Western blotting for the protein of matrix metallopeptidase-2 (MMP-2) and MMP-9 in epidermal growth factor (EGF; 20 ng/ml) or following treatment with EGF (20 ng/ml) plus fisetin (5 and 10 μM) for 24 h. B: Quantitative analysis of western blots. C: Reverse transcription–PCR (RT–PCR) analysis of MMP-2 and MMP-9 mRNA expression. D: Quantitative analysis of RT–PCR. Data are presented as the mean ± standard error of the mean (SEM) of at least three independent experiments. β-actin was used as internal control. **p<0.01 compared with control cells; #p<0.01 compared with fisetin.