Figure 1. Effects of fisetin on EGF in terms of cell viability and migration of ARPE-19 cells. A: ARPE-19 cells were treated with different concentrations of fisetin for 24 h and were tested with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. B: ARPE-19 cells were treated with epidermal growth factor (EGF; 20 ng/ml) or were cotreated with EGF (20 ng/ml) plus different concentrations of fisetin (5, 10, and 20 μM) for 24 h before analysis with the MTT assay. C: In vitro determination of cell migration. D: Microscopy images of ARPE-19 cell migration after scratch (time 0) and after 12 h of EGF, EGF (100 ng/ml) or EGF (100 ng/ml) plus different fisetin (5 and 10 μM) treatments. Quantification of migrating cells is shown as a histogram. Data are presented as the mean ± standard error of the mean (SEM) of at least three independent experiments. **p<0.01 compared with control cells.