Figure 9. Effects of HMGB1 on the expressions of VAP-1, 8-OHdG, and HO-1 in human retinal microvascular endothelial cells. Immunofluorescence
microscopy showed that HMGB1 treatment induced membranous VAP-1 translocation (A), the upregulation of cytoplasmic and nuclear immunoreactivities for 8-OHdG (B), and cytoplasmic immunoreactivity for HO-1 (C; green). Nuclei were counterstained with DAPI (blue).