Figure 1. MEL receptor type 1 (MT₁) and melatonin receptor type 2 (MT₂) in 661W cells. The top left panel shows MT₁ and MT₂ RNA expression. Ladder=100 bp. MT₁ and MT₂ immunoreactivity (green) was detected in 661W cells. Secondary antibody control without a primary antibody (the bottom left panel) and absorption control using a blocking peptide (the bottom center and right panels) were performed. Cell nuclei are shown in red. Scale bar=100 µm.