Figure 1. Dasatinib inhibits VEGF-induced increase in endothelial tube formation, scratch wounding migration, and cell proliferation. A: Tube formation, (B) scratch wounding migration, and (C) cell proliferation assays were performed on HRMECs in the presence or absence of rhVEGF (100 ng/ml) and dasatinib (25 nM). Tube formation, wounding migration, and cell proliferation responses were compared by normalizing the values to those of the corresponding controls. Tube formation and scratch wounding migration were quantified by measuring the tube length and the relative area covered by cells that had migrated from the wound edges (dashed black lines), respectively. Cell proliferation was assessed using the Cell-Counting Kit-8 (CCK-8) assay. Data are presented as the mean ± standard error of the mean (SEM; *p<0.05, ***p<0.001 versus control, ^#p<0.05 versus VEGF only, n=7, scale bars=200 µm).