Appendix 1 of Peng, Mol Vis 2017; 23:8-19.


Appendix 1.

To access the data, click or select the words “Appendix 1.” Morphology and identification of rat bone-marrow mesenchymal stem cells (BM-MSCs). (A) primary BM-MSCs (P0) were cultured for 3 days. The cells grew in a nest shape. The center of the nest was the germinal center. The cells were fibroblast-like. (B) Primary BM-MSCs were cultured for 6 days. The number of cells increased. (C, D) Second-passage (P1) and third-passage (P2) cells. The cell morphology was not changed. (E, F) BM-MSCs were positive for CD44 and CD90 by immunofluorescence. Cells were homogenous, fibroblast-like during the first 3 passages. (G) Fat particles of differentiated adipocytes were stained red with Oil-red O (Methylene blue couterstaining, original magnification 1000×). (H) Calcified nodules of differentiated osteoblasts were stained with Alizarin red.