Figure 1. Flow diagram showing the experimental design. IHC: immunohistochemistry, PBS: phosphate buffered solution; DMEM: Dulbecco’s
Modified Eagle’s Medium; SFM: serum-free medium; FBS: fetal bovine serum; CS: chondroitin sulfate; DMSO: dimethyl sulfoxide;
RT: room temperature; LN2: liquid nitrogen; TEM: transmission electron microscopy.