Figure 4. The impact of ZEB1 reduction on cell migration in HCEnC-21T cells. A: HCEnC-21T cells were transfected with either ZEB1 siRNA-C or scrambled siRNA (sc-siRNA), and knockdown of ZEB1 was validated with western blotting. B: Transfected HCEnC-21T cells were seeded to 100% confluence on an electric cell–substrate impedance sensing (ECIS) electrode
array. A high current (arrow) was applied to the circular electrode underneath the central region of the cell monolayer, thus
clearing the circular area. Migration of cells into the cleared area was monitored for 12 h after wounding by measuring impedance
(ohms) across the electrode at 4,000 Hz. Three wells for each condition were measured; n = 3. Error bars = standard error
of the mean (SEM).