Figure 3. Quantification of DOX-induced apoptosis in ZEB1 knockdown cells. A, B: HCEnC-21T cells were transfected with ZEB1 siRNA-A, ZEB1 siRNA-C, or scrambled siRNA (sc-siRNA), and exposed to DOX for 0, 6, 10, 14, and 18 h. Apoptosis was assessed by the appearance of cleaved caspase 3 (cCASP3) with western blotting. C: ZEB1 knockdown was assessed with western blotting, quantified, and graphed. Abundance of the 14 kDa (D; non-specific), 17 kDa (E; active), and 19 kDa (F) fragments of cCASP3 was quantified with western blotting and graphed. Results represent three independent experiments (n = 3). Error bars = standard error of the mean (SEM).