Figure 1. Cell proliferation in HCEnC-21T cells transfected with siRNA. Cells were transfected with scrambled siRNA (sc-siRNA), ZEB1 siRNA-A, or ZEB1 siRNA-C. A: ZEB1 knockdown was demonstrated by western blotting for ZEB1, with TUBA used as a loading control. B: Cell quantities at 24, 48, and 72 h (N_t) were compared to the initial quantity (N₀), and plotted as the ratio N_t/N₀. Results represent 12 independent data points (n=12). Error bars = standard error of the mean (SEM).