Appendix 3 of Zakharevich, Mol Vis 2017; 23:740-752.


Appendix 3.

To access the data, click or select the words “Appendix 3.” Selection of ZEB1 siRNA molecules was performed by assessing the efficacy of each to knock down ZEB1 protein expression in corneal endothelial cells. HCEnC-21T cells were transfected with ZEB1 siRNA-A, -B and -C, and a scrambled siRNA (sc) was used as a control. Cells were lysed at 24, 48 and 72 h post siRNA transfection and ZEB1 levels were assessed by western blotting. ZEB1 siRNAs A and C demonstrated robust knock down of ZEB1 expression at 48 and 72 h and thus were used for functional assays. TUBA was used as a protein loading control.