Figure 4. P. densiflora bark extract inhibited selenite-induced apoptosis in rat pup lenses. A: The levels of caspase-3, Bcl-2, and Bax mRNA were normalized to the GAPDH mRNA level. B: western blotting analysis indicated protein expression of caspase-3 (35 kDa) and the cleaved form of caspase-3 (17 kDa).
The protein levels were quantified with band density. The results are presented as the mean ± standard deviation (SD) of three
independent experiments (n=4). #p<0.05, ##p<0.01, ###p<0.001 statistically significant different from group 1. *p<0.05, **p<0.01,
***p<0.001 statistically significant different from group 2. Cas-3: caspase-3. Group 1: normal control, Group 2: selenite-treated,
Group 3: selenite/curcumin-treated (80 mg/kg), Groups 4 to 6: selenite/P. densiflora bark extract-treated (40, 80, and 120 mg/kg, respectively) animals.