Figure 3. Interaction of heparanase with RNA polymerase II (Pol II) in high-glucose-induced human retinal endothelial cells (HRECs). The HRECs were incubated with normal medium and high glucose for 72 h. The immunoprecipitated antibody is shown in the top row; the immunoblotted antibody is shown in the bottom row. A: The cell lysates were immunoprecipitated with anti-heparanase antibody and immunoblotted with anti-RNA Pol II antibody. B: The cell lysates were immunoprecipitated with anti-RNA Pol II antibody and immunoblotted with anti-heparanase antibody. The second lane in A and the third lane in B both meet heparanase bound with RNA Pol II together in high glucose HRECs. All experiments were performed in triplicate.