Figure 2. Localizations of heparanase and RNA polymerase II (Pol II) in high-glucose-induced human retinal endothelial cells (HRECs).
The HRECs were cultured in normal media (control: A–D) or treated with high glucose (E–H) for 72 h. Cells were stained with 4',6-diamidino-2-phenylindole (DAPI; blue: A, E), rabbit anti-heparanase antibody (green: B, F), and mouse anti-RNA Pol II antibodies (red: C, G). Low levels of heparanase (B) and RNA Pol II (C) were observed in control HRECs, whereas high levels were observed in the high-glucose cells, with colocalization of heparanase
and RNA Pol II (H, arrows). Western blot analysis indicated that the expressions of heparanase and RNA Pol II were higher in the high-glucose
cells (I).