Figure 5. Nrf2 mediates taxifolin-induced cytoprotective effect against H₂O₂ in ARPE-19 cells. A: RPE cells transfected with si-Nrf2 or with si-NC in the presence of taxifolin. Western blot analysis was performed to measure the levels of Nrf2 using the corresponding antibodies. B: Cell viability and apoptosis were measured in ARPE-19 cells. C: Western blot analysis of cleaved PARP in ARPE-19 cells. Data are shown as the mean ± SD (n = 3); *p<0.05, **p<0.01, and *** p<0.001.