Figure 4 of Xie, Mol Vis 2017; 23:520-528.


Figure 4. Taxifolin increased mRNA and protein expression of nuclear factor (erythroid-derived 2)-like 2 (NRF2), heme oxygenase (HO)-1, NAD(P)H quinine oxidoreductase 1 (NQO1), and glutamate–cysteine ligase modifier (GCLM). A: Cells were treated with 10, 20, and 50 µM of taxifolin for 24 h. Western blot analysis was performed using the corresponding antibodies. B: Total RNA was extracted, and the amounts of NRF2, HO-1, NQO1, and GCLM mRNA were quantified with real-time PCR and normalized to the corresponding amounts of β-actin mRNA. Data are shown as mean ± SD (n = 3); *p<0.05, **p<0.01, and *** p<0.001.