Table 2 of
Martins, Mol Vis 2017; 23:495-503. Table 2 of
Martins, Mol Vis 2017; 23:495-503.
Table 2. Details of the previous molecular screening of LHON by RFLP-PCR.
| Mutation | Primers (5′ >3′) | Endonuclease | Fragments | |
|---|---|---|---|---|
| Wild Type | Mutant | |||
| m.G11778A | F: AACTACGAACGCACTCACAG R: GAAGTCCTTGAGAGAGGATTA | SfaNI | 17/32bp | 49bp |
| m.T14484C | F: GTAGTATATCCAAAGATAACCA R: CTTCTAAGCCTTCTCCTA | BsaBI | 19/135bp | 154bp |
| m.G3460A | F: GGCTACTACAACCCTTCGC R: GGCTCTTTGGTGAAGAGTTTT | BsaHI | 22/27bp | 49bp |