Table 1 of Martins, Mol Vis 2017; 23:495-503.

Table 1. Customized OpenArray™ assays.

Assay Primer (5′ >3′) Amplified sequence
m.G11778A F: TGCCTAGCAAACTCAAACTACGAA
R: GGGAGTAGAGTTTGAAGTCCTTGAG TGCCTAGCAAACTCAAACTACGAACGC ACTCACAGTC[G/A]CATCATAATCCTCTCTCAAGGACTTCAAACTCTACTCCC
m.T14484C F: TCCTCAATAGCCATCGCTGTAGTAT
R: CGGGTGTGTTATTATTCTGAATTTTGGG TCCTCAATAGCCATCGCTGTAGTATATCCAAAG ACAACCA[T/C]CATTCCCCCTAAATAAATTAAAAAAACTATTAAACCCATATAACCTCCCCCAAAATTCAGAATAATAACACACCCG
m.G3460A F: CCCCTACGGGCTACTACAAC
R: GATGGTAGATGTGGCGGGTTT CCCCTACGGGCTACTACAACC CTTCGCTGAC[G/A]CCATAAAACTCTTCACCAAAGAGCCCCTAAAACCCGCCACATCTACCATC

F: Forward; R: Reverse. Underline/bold sequence: Customized TaqMan® probe. Inside the brackets, the first nucleotide indicates the normal sequence, dyed with VIC, and the second, the mutant allele, dyed with FAM. For each assay were customized a pair of primers and a pair of probes (bold and underlined).

Martins, Mol Vis 2017; 23:495-503. http://www.molvis.org/molvis/v23/495

©2017 Molecular Vision http://www.molvis.org/molvis/

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