Table 1 of
Santhanam, Mol Vis 2017; 23:39-51. Table 1 of
Santhanam, Mol Vis 2017; 23:39-51.
Table 1. PCR primers designed for LCM-RT–PCR.
| Gene | Primer sequences (5’-3’) | Size (bp) | Annealing (°C) | GenBank accession |
|---|---|---|---|---|
| NID1 | F: ATGGGTTCAGCATCACTGGG R: TAATGACCAGCTTGCCTGGG | 81 | 58 | XM_017347600.1 |
| NID2 | F: CGTGCAAGACACGGAAGTCA R: GAGTTGGCTGGGACGTAAGG | 141 | 58 | XM_008269653.2 |
| HSPG2 | F: CGTGGCAGTCAACACCAAAG R: TTCTTGATGCAGCCCGTGAT | 105 | 58 | XM_017346486.1 |
| LAMA3 | F: CTCCCAAATGCCTCGTTCCT R: TTTGTACCGCACCCTGAGAC | 138 | 58 | XM_002713604.1 |
| KERA | F: AGTGCGGATGACTTTGATTG R: TGGCTTCTCTGGAATGGTTT | 165 | 58 | XM_002711384.3 |
| KRT12 | F: GGCTCTCTGTATCTCCCCTCT R: CCATTTCCACGCTGACCT | 541 | 59 | XM_002719366.3 |
| KRT3 | F: ATGTCCGTGGTGCTGTCC R: GCTGGCGTTCTGCTTCTT | 267 | 60 | XM_002711005.2 |
| ACTB | F: CACCCTCTCTCGACGAAACC R: CTGTCACCTTCACCGTTCCA | 152 | 60 | NM_001101683.1 |