Figure 4. Genistein-induced miR-145 directly targets ABCE1. A: Putative seed-matching sites or mutant sites (red) between miR-145 and the 3′-untranslated region (UTR) of ABCE1. B: Luciferase reporter assay was performed on Y79 cells to detect the relative luciferase activities of wild-type (WT) and mutant ABCE1 reporters. ** p<0.01. C–D: Y79 cells were transfected with miR-145 or control miR-NC mimics. After 48 h, the expression levels of ABCE1 were analyzed with quantative reverse transcription (qRT)-PCR and western blotting. ** p<0.01 indicates a statistically significant difference compared with the miR-NC group. E–F: Y79 cells were transfected with anti-miR-145 inhibitor (anti-miR-145), or control anti-sense RNA (anti-miR-NC), and were treated with 50 μM genistein or dimethyl sulfoxide (DMSO). After 48 h, the expression levels of ABCE1 were analyzed with qRT-PCR and western blotting. **p<0.01 indicates a statistically significant difference compared with the anti-miR-NC + DMSO group. ##p<0.01 indicates a statistically significant difference compared with the anti-miR-NC + genistein group.