Figure 3. Silence of miR-145 reverses the suppression of genistein in the retinoblastoma. A: Y79 cells were transfected with the anti-miR-145 inhibitor (anti-miR-145) or control anti-sense RNA (anti-miR-NC) and were treated with 50 μM genistein or dimethyl sulfoxide (DMSO). After 48 h, the expression levels of miR-145 were analyzed with qRT-PCR. B: Cells were treated as above, and a Cell Counting Kit-8 assay was used to detected cell viability. C: Cloning efficiency was measured by the number of clones growing in soft agar. D: Cell apoptosis was analyzed with flow cytometry as described. Data are presented as mean + standard deviation (SD) from three independent experiments with triple replicates per experiment. **p<0.01 indicates a statistically significant difference compared with the anti-miR-NC + DMSO group. ##p<0.01 indicates a statistically significant difference compared with the anti-miR-NC + genistein group.