Figure 3. SERPINE2 transcript in ocular tissue. A: Reverse-transcription PCR (RT–PCR) of SERPINE2 mRNA levels detected in the murine RPE, the murine retina, human ARPE-19 cells, and the human retina. Products were resolved in a 2% agarose gel and stained with ethidium bromide. A photo of the gel exposed to ultraviolet (UV) light is shown with DNA size markers (lane 1), and the cDNA templates for the reactions in the murine RPE (lane 2), the murine retina (lane 3), human ARPE-19 cells (lane 4), and the human retina (lane 5). The migration positions for some markers are indicated to the left. B: Schematics of the primer constructs used for PCR. The expected number of nucleotides for the product from the transcript NM_009255.4 encoding Mus musculus serine (or cysteine) peptidase inhibitor, clade E, member 2 (Serpine2), mRNA using the murine PN-1 primers is indicated. The expected number of nucleotides for the product from the transcript NM_006216.3 encoding Homo sapiens serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2 (SERPINE2), transcript variant 1, mRNA using the human PN-1 primers is indicated. Mu, murine; Hu, human; bp; base pairs.