Figure 1. Impact of MFA on spontaneous and response firing. A: Example raw traces of extracellular activity showing the same channel under control (artificial cerebrospinal fluid [aCSF]) and meclofenamic acid (MFA) conditions. B: Power spectral density (PSD) of spiking in (A). C-D: Bar graphs representing the average (± standard error of the mean [SEM]) raw overall power (C) and spontaneous firing rate (D) of all recorded retinal ganglion cells (RGCs); n = 417 cells. E: Distribution of the peak frequencies of all recorded RGCs crossing a normalized power threshold of 9%. Insert: Fraction of RGCs with a maximum normalized power value exceeding 9%; n = 9 retinas. F-G: Retinal section from adult C3H rd1 mouse treated with adenoassociated virus (AAV2)-grm6-Rho and fluorescently tagged for rhodopsin (F) and nucleic acid (G). The signal from the rhodopsin antibody is present in the outer portion of the inner nuclear layer (INL), where the cell bodies of ON bipolar cells reside [32]. H-K: Example trial bin counts (TBCs; H, J) and peri-stimulus time histogram (PSTH; J, K) of cells in aCSF (H, I) and MFA (J, K) conditions responding to a 2 s stimulus (epoch indicated by dotted box and square-wave diagrams) at an irradiance of 10¹³ photons/cm²/s from a dark background. L-M: Example TBC (L) and PSTH (M) of the melanopsin-mediated light response in the untreated retina to a stimulus of irradiance of 10¹⁴ photons/cm²/s. DAPI = 4',6-diamidino-2-phenylindole; GCL = ganglion cell layer; IPL = inner plexiform layer. ** p= 0.0078, **** p<0.0001.