Table 2 of Fang, Mol Vis 2017; 23:306-317.

Table 2. Patients with positive results for bacteria as determined by microscopy, culture, PCR, and/or the bacterial dot assay.

      Case Age Sex Microscopya Culture PCRd       Bacterial dot assay
      no.       probe BP1       probe BP2       probe BP3       probe Psu
      True positive cases by the probe BP3
      2       25       F       GNBb       P. aeruginosa       +       −       −       +       +
      18       57       F       Yeast       Glucose non-fermenter; mold       −       −       −       +       −
      20       57       F       NDc       M. fortuitum       −       +       +       +       −
      23       62       M −       P. aeruginosa; S. epidermidis       +       +       +       +       +
      26       42       M −       P. aeruginosa       +       +       +       +       +
      27       63       M −       P. aeruginosa       +       +       +       +       +
      28       75       M −       P. aeruginosa       −       +       +       +       +
      29       41       F −       P. aeruginosa       −       +       +       +       −
      32       58       M       GNB       P. aeruginosa       +       +       +       +       +
      33       74       F       GNB       P. aeruginosa       +       +       +       +       +
      35       43       M       −       P. aeruginosa       +       +       +       +       +
      39       56       F       ND       P. aeruginosa       +       +       +       +       +
      42       69       M       GNB       P. aeruginosa       +       +       +       +       +
      45       30       M       GNB       P. aeruginosa       +       +       +       +       +
      50       56       M       −       P. aeruginosa       +       +       +       +       +
      False negative cases by the probe BP3
      13       63       M       ND       S. haemolyticus       +       −       −       − −
      False positive cases by the probe BP3
      3       59       M       −       Mold       −       +       +       + −
      17       77       M       Yeast       Yeast       −       +       +       + −
      22       72       M       ND       Yeast       −       +       +       + −
      49       65       F       −       Mold       −       +       +       + −
      Cases with true negatives by the probe BP3 but false negatives by the probes BP1 or BP2
      9       50       F       −       Mold       −       +       −       −       −
      19       51       F       −       −       −e       +       −       −       −
      21       69       F       ND       −       −       +       −       −       −
      24       71       M       −       Candida spp.       −       +       +       −       −
      25       62       M       ND       −       −       +       −       −       −
      30       72       M       −       −       −       +       −       −       −
      31       20       M       −       Acanthamoeba spp.       −       +       −       −       −
      36       47       M       ND       −       −       +       −       −       −
      37       27       M       ND       −       −       +       −       −       −
      38       74       M       −       −       −f       +       −       −       −

aMicroscopy examination by Gram stain and acid fast stain. bGNB, gram-negative bacilli. cND, not done. dPCR, polymerase chain reaction for bacteria. eNegative PCR result for bacteria but positive PCR result for microsporidia. fNegative PCR result for bacteria but positive PCR result for herpes simplex virus.

Fang, Mol Vis 2017; 23:306-317. http://www.molvis.org/molvis/v23/306

©2017 Molecular Vision http://www.molvis.org/molvis/

ISSN 1090-0535