Figure 2. Immunohistochemical evaluation of expression of retinal markers in 12-month-old WNIN/GR-Ob rats. The expression value of each bar was compared to its respective age-matched lean rat which is taken as 1 (or 100%) for calculating the fold change. A-F: rhodopsin, G-L: glial fibrillary acidic protein (GFAP), and M-R: vascular endothelial growth factor (VEGF). Nuclei are labeled with 4,6-diamidino-2-phenylindole (DAPI). Scale bar, 50 µm.