Figure 2. RAC1 regulates CTGF, LOX, and miR-21 expression in hyperglycemic condition. Perindopril-treated cells were transfected with 20 nM of control (Scr) siRNA or RAC1 siRNA in the presence of high glucose and harvested for miRNA-enriched total RNA to measure CTGF and LOX (A) and miR-21 (B) expression as quantified with quantitative real-time PCR (qRT-PCR). Untransfected cells treated with NG were also used as control to high glucose. The level of each transcript was normalized to that of HPRT/snRNA U6. The ratio was then normalized to that of control. Values are presented as mean ± standard error of the mean (SEM); n = 4; *p<0.05, versus NG; #p<0.001 versus scramble siRNA (high glucose) or (2B); $p = 0.001 versus scramble siRNA (high glucose).